rabbit anti par1  (Bioss)

Journal: Heliyon

Article Title: The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells ☆

doi: 10.1016/j.heliyon.2024.e33329

Figure Lengend Snippet:

Article Snippet: Rabbit anti PAR1 , MyBiosource, MBS9201361 , –.

Techniques: Recombinant, Real-time Polymerase Chain Reaction, Software

Journal: Heliyon

Article Title: The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells ☆

doi: 10.1016/j.heliyon.2024.e33329

Figure Lengend Snippet: The effect of PAR1 modulation on cell-cycle and cell proliferation in C6 cells: A. Quantification of distribution in the different phases of the cell cycle in control and thrombin (1U/ml) treated C6 cells, N = 5, t -test. B. Quantification of distribution in the different phases of the cell cycle in control and PAR1 antagonist (100 nM) treated C6 cells. N = 5, t -test. C. Thrombin activity in the medium in various cell densities. N = 10, Kruskal-Wallis test. D. Thrombin activity per cell in various cell densities. E. Cell proliferation following combinations of thrombin (1U/ml) and PAR1 antagonist (100 nM) treatments. N = 24, 30 and 36, one-way ANOVA with Tukey's post-hoc analysis. Results are presented as mean ± SEM. *p < 0.03, ***p < 0.0008, ****p < 0.0003.

Article Snippet: Rabbit anti PAR1 , MyBiosource, MBS273633 , –.

Techniques: Control, Activity Assay

Journal: Heliyon

Article Title: The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells ☆

doi: 10.1016/j.heliyon.2024.e33329

Figure Lengend Snippet: PAR1 modulation effect on process and cell migration: A. Representative α-tubulin (green) staining of C6 control, PAR1 antagonist (100 nM) and thrombin (1U/ml) treated cells. Scale bar – 10 μm. B. Process length in control, PAR1 antagonist and thrombin treated C6 cells. N = 20, Kruskal-Wallis test with Dunn's post-hoc analysis. C. Number of process per cell control, PAR1 antagonist and thrombin treated C6 cells. N = 17, Mann-Whitney test. D. Percent of wound closure following PAR1 antagonist (100 nM) and thrombin (1U/ml) treatments. N = 13 and 16. t -test. E. Representative images of wound closure in untreated, PAR1 antagonist and thrombin treated cells at t = 0 h (upper panel) and t = 3 h (lower panel). Results are presented as mean ± SEM. *p = 0.03, **p = 0.0094, ****p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Rabbit anti PAR1 , MyBiosource, MBS273633 , –.

Techniques: Migration, Staining, Control, MANN-WHITNEY

Journal: Heliyon

Article Title: The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells ☆

doi: 10.1016/j.heliyon.2024.e33329

Figure Lengend Snippet: mRNA expression following PAR1 modulation: PAR1 (A), FX (B) EB3 (C) and EB1 (D) mRNA expression in control, PAR1 antagonist (100 nM) and thrombin (1U/ml) treated C6 cells. N = 3 to 7. Results are presented as mean ± SEM. Using one-way ANOVA with post-hoc analysis. Results for EB3 were analyzed using t -test. *p < 0.035, **p = 0.006.

Article Snippet: Rabbit anti PAR1 , MyBiosource, MBS273633 , –.

Techniques: Expressing, Control

Journal: Heliyon

Article Title: The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells ☆

doi: 10.1016/j.heliyon.2024.e33329

Figure Lengend Snippet: The effect of PAR1 antagonist on comet length, track length and duration. A. Representative live imaging of N1E-115 cells transfected with RFP-EB3 (red) in control and PAR1 antagonist (100 nM) treated cells. Quantification of comet length (B), track length (C) and truck duration (D) in control and PAR1 antagonist (100 nM) treated cells. N = 57 to 60, t -test. Results are presented as mean ± SEM. *p < 0.035, **p = 0.006. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Rabbit anti PAR1 , MyBiosource, MBS273633 , –.

Techniques: Imaging, Transfection, Control

Journal: Heliyon

Article Title: The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells ☆

doi: 10.1016/j.heliyon.2024.e33329

Figure Lengend Snippet: PAR1-tubulin α co-localization: Representative images of C6 cells stained to α-tubulin (red), PAR1 (green) and Hoechst (blue) in different stages of mitosis. A-D. Metaphase, r = 0.953. E-H. Early anaphase, r = 0.867. I-L. Late anaphase, r = 0.953. M-P. Telophase, r = 0.864. Scale bar – 10 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Rabbit anti PAR1 , MyBiosource, MBS273633 , –.

Techniques: Staining

Journal: Heliyon

Article Title: The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells ☆

doi: 10.1016/j.heliyon.2024.e33329

Figure Lengend Snippet:

Article Snippet: Rabbit anti PAR1 , MyBiosource, MBS273633 , –.

Techniques: Recombinant, Real-time Polymerase Chain Reaction, Software

Journal: Heliyon

Article Title: The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells ☆

doi: 10.1016/j.heliyon.2024.e33329

Figure Lengend Snippet:

Article Snippet: Rabbit anti PAR1 , MyBiosource, MBS273633 , –.

Techniques: Recombinant, Real-time Polymerase Chain Reaction, Software

Journal: International Journal of Molecular Sciences

Article Title: Endothelial Protein C Receptor and 3K3A-Activated Protein C Protect Mice from Allergic Contact Dermatitis in a Contact Hypersensitivity Model

doi: 10.3390/ijms25021255

Figure Lengend Snippet: The expression/activity of EPCR, PC/aPC, PAR1, and PAR2 in the skin of normal and CHS mice. ( A ) Representative images of EPCR, PC/aPC, PAR1, and PAR2 expression in skin tissues of WT and EPCRKO mice (8 weeks old, flank skin), detected by immunochemistry. ( B ) Representative images of EPCR, PC/aPC, PAR1, and PAR2 expression in ear tissues of WT and EPCRKO mice with CHS at day 14. ( C ) Representative images of EPCR, PC/aPC, PAR1, and PAR2 expression in skin tissues of WT mice treated with 3K3A-aPC (1 mg/kg). Scale bars in ( A – C ): 100 µm. ( D ) The staining intensity of EPCR, PC/aPC, PAR1, and PAR2 in the skin epidermis in ( A – C ), expressed as means ± SD (n = 8). ( E ) aPC activity in the ear skin tissues/plasma, expressed as means ± SD (n = 8 for all groups), against human recombinant aPC. * p < 0.05. ** p < 0.01.

Article Snippet: Mouse skin tissue sections were de-paraffinized, heat-retrieved, and immunostained using rabbit anti-mouse PAR1 and goat anti-mouse PAR2 antibodies (Santa Cruz Biotech, Dallas, TX, USA), goat anti-mouse EPCR and rabbit anti-mouse PC/aPC antibodies (R&D Systems).

Techniques: Expressing, Activity Assay, Staining, Clinical Proteomics, Recombinant